N7892 Sigma

Nitrocellulose membranes for blotting

pore size 0.2 μm, size 15 cm × 15 cm

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Description

Application

Nitrocellulose membranes are the most popular membranes for immunoblotting (Western blotting) of proteins. The basis of protein binding to nitrocellulose is believed to be due to hydrophobic interactions. The 0.45 μm pore size is the most commonly used for proteins greater than 10,000 MW, but for smaller proteins 0.2 μm is recommended to more efficiently trap the proteins.1

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