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N8264 Sigma

Nucleoside Phosphorylase from microorganisms

lyophilized powder, ≥10 units/mg protein

Synonym: Nucleoside Phosphorylase bacterial, PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

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Description

Application

Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with xanthine oxidase (XTO-212) and uricase (UAO-201, UAO-211). Purine nucleoside phosophorylase has shown the ability to perform both phosphorylosis and synthesis of purine deoxy- and ribonucleosides. It has also been found that membrane-associated nucleoside phosphorylases may have a transmembranal orientation with their base and ribose-1-P binding sites on opposite sides of the membrane.

Biochem/physiol Actions

Catalyzes the following reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

General description

Nucleoside phosphorylase is an enzyme important for the biosynthesis of nucleosides.

Packaging

100, 250 units in glass bottle

500, 1000 units in poly bottle

Unit Definition

One unit will cause the phosphorolysis of 1.0 μmole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.4 at 25 °C.

Physical form

Lyophilized powder containing potassium gluconate, mannitol and EDTA

Physical properties

Isoelectric point : 4.1 +/- 0.1
Michaelis constants : 6.4 x 10-5M (Inosine), 3.2x10-4M (Pi)
Inhibitors : p-Chloromercuribenzoate, SDS, Hg++, Ag+Optimum pH : 7.5 - 8.0
Optimum temperature : 65oC
pH Stability : pH 6.0 - 9.0 (30oC, 16hr)
Thermal stability : below 60oC (pH 7.7, 30min)

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Assay Procedure for Nucleoside Phosphorylase Bacterial

One unit causes the formation of one micromole of uric acid per minute under the conditions described below.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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