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N8630 Sigma

Nuclease P1 from Penicillium citrinum

lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)

Synonym: Endonuclease P1

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
form   lyophilized powder
secondary activity   ≥1,000 units/mg protein 3′-nucleotidase
packaging   vial of ≥250 units (using RNA substrate)
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.

General description

Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea.

Unit Definition

3′-5′-Phosphodiesterase: One unit will liberate 1.0 μmole of acid soluble nucleotides from RNA per min at pH 5.3 at 37 °C.
3′-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3′-AMP per min at pH 7.2 at 37 °C.

Application

Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA.

Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.

The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.

Physical properties

A zinc dependent glycoprotein consisting of 270 amino acid residues. Molecular mass: 42-50 kDa.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

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15

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