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N9280 Sigma

All-in-One Nick Translation Labeling Mix −dATP

In Strips of Eight 0.2 ml Tubes

Synonym: All-in-One Nick Translation Labeling Mixes

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Properties

form   lyophilized powder
storage temp.   2-8°C

Description

Features and Benefits

• Achieve a specific activity of 108 dpm/μg in 30 minutes
• Label template amounts ranging from 10 ng to several μg
• Label templates ranging from small restriction fragments to lambda or cosmid DNA
• Produce radioactive or non-radioactive probes (digoxigenin, fluorescein, biotin, or rhodamine)
• DNA templates may be supercoiled or linearized

Other Notes

Color coded blue

Application

All-In-One Nick Translation Labeling Mix produces radioactive or non-radioactive DNA probes for use in a variety of hybridization techniques, including Northern, Southern, colony/plaque lift, and dot blotting applications. Each single-use tube contains all of the necessary reaction components, including DNase I and Escherichia coli DNA Polymerase I (Klenow fragment), three deoxynucleotides, and reaction buffer. The All-In-One Labeling Mix is provided as a room temperature stable, static-free, and quick dissolving lyophilized solid. The labeling mix is available it two formats, without dATP or without dCTP. For added convenience, All-In-One Labeling Mixes are provided in color-coded 0.2- or 1.5-ml tube formats for quick identification.
The All-In-One Labeling Mix is easy to use. Simply prepare the DNA in 1X Tris-EDTA (TE) buffer, add to the lyophilized labeling mix, add in the labeled nucleotide, mix, and incubate at 15°C for as little as 30 minutes. Stop the reaction with EDTA and remove any unincorporated, labeled nucleotide by standard techniques. The labeled probe is now ready for use in a variety of hybridization techniques. Each package contains a vial of Lambda DNA Hind III digest as a control template and is provided with a detailed technical bulletin.

Legal Information

All-in-One is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
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