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N9284 Sigma

Nitroreductase from Escherichia coli

≥90% (SDS-PAGE), recombinant, expressed in E. coli

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Properties

Related Categories 1.x.x.x Oxidoreductases, 1.x.x.x Others, Biochemicals and Reagents, Chemical Synthesis, Enzyme Class Index,
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
mol wt   monomer mol wt 24000
greener alternative category   Re-engineered
shipped in   wet ice
storage temp.   −20°C
Gene Information   Escherichia coli K12 ... nfsB(945483)

Description

General description

Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.

Preparation Note

Produced using animal component-free materials.

Unit Definition

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.

Application

Nitroreductase has been used in a study that used a set of PCR primers to clone a gene encoding a hypothetical nitroreductases (named as Ssap-NtrB) from uropathogenic staphylococcus. It has also been used to improve prodrug activation. Nitroreductase from Escherichia coli has been used in a study to assess anaerobic bacteria as a gene delivery system for cancer treatment. It has also been used in a study to investigate its applications in antibody-directed enzyme prodrug therapy.

Biochem/physiol Actions

Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.

Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Physical properties

Lyophilized powder containing PBS. Does not contain BSA as excipient

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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