Synonym: p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
|Related Categories||p-Nitrophenyl Phosphate Substrate Systems, Alkaline Phosphatase, Alphabetical Listing, Biochemicals and Reagents, Colorimetric,|
|description||5 mg substrate per tablet|
|fluorescence||λex 310 nm (pH 7.1)|
|λex 399 nm (Reaction Product)|
Chromogenic substrate for the determination of acid and alkaline phosphatases.1,2 The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
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powder, BioReagent, suitable for cell culture, ≥97%
for enzyme immunoassay, ≥99.0% (enzymatic)
ready to use solution
Certificate of Analysis
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
1. J.-P. Bretaudiere and T. Spillman, H.U. Bergmeyer, ed. Methods of Enzymatic Analysis 3rd ed., Weinheim, Fed.Rep.Ger. 4, 75, (1984)
2. O.H. Lowry Meth. Enzymol. 4, 366, (1957)
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