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N9527 Sigma

Monoclonal Anti-NTF2 antibody produced in mouse

~2 mg/mL, clone 4F5, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Nuclear Transfer Factor 2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Nuclear Proteins, Epigenetics,
species reactivity   human, hamster, rat, mouse, monkey, Xenopus, chicken
application(s)   immunocytochemistry1: suitable using 3.7% formaldehyde-0.2% Triton X-100 or 4% paraformaldehyde-methanol.
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using HeLa cells cytosol preparation
clone   4F5, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
mol wt   antigen mol wt 14 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NUTF2(10204)
mouse ... Ntf2(104255)
rat ... Nutf2(291981)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human NTF2. The epitope lies within the extreme C-terminus of NTF2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

The antibody may also be used for microinjection, in vitro nuclear protein import assays, and blocking of NTF2 binding to Ran.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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