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  • N9528 - Anti-Neuropeptide Y (NPY) antibody produced in rabbit

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N9528 Sigma

Anti-Neuropeptide Y (NPY) antibody produced in rabbit

whole antiserum

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies for Obesity Research,
species reactivity   rat, pig, human, sheep
application(s)   immunohistochemistry (frozen sections): 1:8,000-1:16,000 using 4% paraformaldehyde perfusion-fixed sections of rat brain
  indirect ELISA: 1:5,000-1:10,000 using using peptide coating at 0.5-1 μg/well in 0.05 M carbonate/bicarbonate buffer, pH 9.6, and pNPP as substrate
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Npy(24604)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic neuropeptide Y (NPY, porcine), conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in human and rat NPY and is highly conserved (84% identity) in the C-terminus region (24-36) of human peptide YY.

General description

Neuropeptide Y (NPY), a 36-amino acid peptide amide, is a major regulatory neuropeptide widely distributed in the mammalian central (CNS) and peripheral nervous systems (PNS). NPY belongs to the pancreatic polypeptide family of peptides which are characterized by a common tertiary structure. Within this family, an intestinal peptide hormone, peptide YY (PYY), is most closely related to NPY. In the CNS, NPY is involved in regulation of blood pressure, memory processing, circadian rhythm, and stimulation of food intake. In the PNS, NPY has potent vasoconstrictor activity and acts as a neurotransmitter/neuromodulator of sympathetic neurons and adrenal glands. NPY is one of the most abundant peptides found in the CNS, widely distributed in the brain. High levels of NPY are present in the cerebral cortex, amygdaloid nuclei, hippocampal formation, and hypothalamus. In the PNS, NPY is found mainly in sympathetic neurons that innervate vascular smooth muscle, heart, and urogenital tract. The biological actions of NPY in the brain and periphery are mediated by at least two different NPY receptors, designated Y1 and Y2 receptor subtypes.

Specificity

The antibody recognizes by ELISA, NPY (human), NPY (porcine), NPY (sheep), NPY (13-36), NPY (18-36), and cross-reacts with PYY (human). It does not cross-react with neurokinin B (NKB), neurokinin A (NKA), substance P (SP), calcitonin, somatostatin and BSA.

Application

The antibody may be used to detect Neuropeptide Y (NPY) by various immunochemical techniques such as ELISA and immunohistochemistry (IHC).
ELISA indirect: a working antibody dilution of 1:5,000-1:10,000 is determined using a peptide coating at 0.5-1 mg/well in 0.05 M carbonate/bicarbonate buffer, Catalog Number C3041, pH 9.6, and pNPP, Catalog Number N2770. as substrate.
Immunohistochemistry: a working antibody dilution of 1:8,000-1:16,000 is determined using frozen sections of rat brain perfusion-fixed with 4% paraformaldehyde.

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