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  • N9539 - Anti-Nedd9 (C-terminal region) antibody produced in rabbit

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N9539 Sigma

Anti-Nedd9 (C-terminal region) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CAS2, Anti-CASL, Anti-CASS2, Anti-CRK-associated substrate-related protein, Anti-HEF1

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
species reactivity   human
application(s)   indirect immunofluorescence: 1.5-3.0 μg/mL using human HS68 cells.120
  western blot: 1-2 μg/mL using Jurkat cell lysates
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~120 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NEDD9(4739)
mouse ... Nedd9(18003)
rat ... Nedd9(291044)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 672-691 of human Nedd9, conjugated to KLH. The corresponding sequence is identical in rat and mouse Nedd9.

General description

NEDD9 (neural precursor cell expressed, developmentally down-regulated 9) is a non-catalytic multi-domain scaffolding protein. The gene contains retinoic acid response element (RARE) element in its promoter region, and is composed of seven exons. It encodes for a protein composed of 834 amino acids in humans, and contains an N-terminal SH3 (src homology 3) domain, followed by SD - substrate domain, SRR - serine rich region, FAT - focal adhesion targeting domain. Its C-terminal contains a DYDY motif (amino acids 628–631). This gene is expressed ubiquitously, and shows strong expression in lungs and lung cell lines. NEDD9 is a member of the Cas (Crk-associated substrate) family of scaffolding proteins.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

NEDD9 (neural precursor cell expressed, developmentally down-regulated 9) functions in the assembly of oncogenic kinase complexes such as focal adhesion kinase (FAK), ABL, SRC, Aurora-A (AURKA). It, therefore, controls signaling pathways implicated in processes such as cell cycle, migration, adhesion, invasion and chemotaxis that are involved in for tumorigenesis and metastases. In melanoma, glioblastoma, colon cancer and head and neck squamous carcinoma, the overexpression of this protein results in enhanced epithelial to mesenchymal transition, migration, and metastasis.

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