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N9657 Sigma

Monoclonal Anti-Nitric Oxide Synthase, Inducible antibody produced in mouse

clone NOS-IN, ascites fluid

Synonym: Anti-iNOS

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
species reactivity   rat, mouse
application(s)   microarray: suitable
  western blot: 1:1,000 using whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-γ
clone   NOS-IN, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 130 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NOS2(4843)
mouse ... Nos2(18126)
rat ... Nos2(24599)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) from mouse macrophage (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS.

General description

On the basis of molecular mass, subcellular location, and Ca2+ dependence, at least three types of NOS have been classified. Type I NOS is found in neurons. It is a 150-160 kD protein, also called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+-regulated NOS (cNOS). Type II, best characterized in macrophages, is a 130 kDa protein, also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III is found in endothelial cells. It is a 135 kDa protein, also called endothelial NOS (eNOS, or ecNOS). Neuron and endothelial NOS are constitutively expressed and are dependent on Ca2+calmodulin for NO production, whereas Type II NOS is Ca2+ -independent and is expressed in activated macrophages and some glial cells after stimulation. Nevertheless, evidence indicates that the various types of NOS may serve a variety of diverse biological pathways. For instance, iNOS is not found only in macrophages but also in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts.

Specificity

Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS), antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) may be used for the localization of inducible type nitric oxide synthase (iNOS), using various immunochemical assays such as ELISA, immunoblot,and immunocytochemistry.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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