GenElute™ HP Plasmid Miniprep Kit
sufficient for 350 preparations
DOWNLOAD MSDS (PDF)Synonym: Gen Elute, GenElute™ HP Plasmid Kit
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Properties
| Related Categories | Core Bioreagents, DNA & RNA Purification, Kits, Life Science Reagents for Nucleic Acid Purification, Molecular Biology, |
| usage | sufficient for 350 preparations |
Description
Features and Benefits
• Purified plasmid DNA in less than 30 minutes
• Up to 25 μg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• No phenol/chloroform extraction or alcohol precipitation required
Application
The recovered plasmid DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transcription, and sequencing.
General description
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.
The GenElute™ HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 mg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
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sufficient for 250 purifications
sufficient for 70 preparations
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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