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GenElute HP Plasmid Maxiprep Kit Green Alternative

sufficient for 10 purifications

Synonym: GenElute HP Plasmid Maxiprep Kit

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, DNA & RNA Purification, Greener Alternative Products, Life Sciences,
usage   sufficient for 10 purifications
greener alternative product characteristics   Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

Description

Features and Benefits

• From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
• Up to 1.2 mg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• Contains fewer plastic components than other high speed kits, reducing the amount of waste
• No phenol/chloroform extraction or alcohol precipitation required

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
77 °F
Flash Point(C) 
25 °C
Protocols & Articles

Articles

Plasmid DNA Purification

Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a ...
Keywords: Centrifugation, Chromatography, Cloning, DNA purification, Digestions, Genetic, Ion Exchange, Molecular biology, Nucleic acid denaturation, Nucleic acid purification, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transcription, transformation

Related Content

GenElute™ HP Plasmid Maxiprep Procedure [VIDEO]

The GenElute HP Plasmid Maxiprep kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cutures. The recovered plasmid DNA is ready for immediate use...
Keywords: Digestions, Peptide synthesis, Polymerase chain reaction, Sequencing, Transfection, transformation

Peer-Reviewed Papers
15

References

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