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NA1020 Sigma

GenElute PCR Clean-Up Kit

sufficient for 70 purifications

Synonym: Gen Elute, PCR Purification

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Application

GenElute PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.

Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Features and Benefits

• Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
• Recovers up to 95% of PCR products between 100 bp and 10 kb
• Removes over 99% of primers and other components
• Eliminates the need to remove mineral oil by organic extraction
• 40% more purification preps supplied than market leader

Principle

The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).

General description

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Can Product NA1020, GenElute™ PCR Clean-Up Kit, be used for isolation of PCR products labeled with digoxigenin?
Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use
Can Product No. NA1020, GenElute™ PCR Clean-Up Kit,  be used to clean up PCR products larger than 10 kb?
We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well.  That said, the bound DNA might be more difficult to elute.  Heating the elution solution to 60-65°C may help with elution.  Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.
When using Product No. NA1020, GenElute™ PCR Clean-Up Kit, what is the largest amount of PCR reaction sample that can be processed on one column?
The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.
When using GenElute™ PCR Clean-Up Kit, Product No. NA1020, Can the amount of elution buffer be reduced in order to obtain a more concentrated eluate?
One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 µl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.
Can GenElute™ PCR Clean-Up Kit, Product NA1020, be used for isolation of labeled PCR products?
This kit will work with labeled PCR products and no modifications to the standard protocol are needed.
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Peer-Reviewed Papers
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References

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