Creating Transgenic Mice using CRISPR-Cas9 Genome Editing
While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot
Extract-N-Amp™ Plant Tissue PCR Kits
Description Procedure Application Features and Benefits Order Information Kit Contents Sample Data Materials
Keywords: Amplification, DNA purification, Digestions, Phase transitions, Polymerase chain reaction, Precipitation, Purification
Plasmid DNA Purification Overview
Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a ...
Keywords: Centrifugation, Chromatography, Cloning, DNA purification, Digestions, Genetic, Ion Exchange, Molecular biology, Nucleic acid denaturation, Nucleic acid purification, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transcription, transformation
Transplex Whole Transcriptome RNA Amplifications
The efficacy of amplification of small quantities of total RNA with the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study. Total RNA extracted from decreasin...
Ken Heuermann, Brian Ward
Biowire Volume 10 Article 1
Keywords: AGE, Amplification, Applications, Cancer, Clinical, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genomics, Instructions, Marketing, Methods, Microarray Analysis, Microbiology, Nucleic acid annealing, Nucleic acid hybridization, Oligonucleotide microarrays, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Reductions, Transcription
Blood Card - Extraction & Amplification WGA Protocol
Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream...
Complete Whole Transcriptome Amplification Kit WTA2 Protocol
Product Description Components Storage/Stability Procedure Troubleshooting Guide Frequently Asked Questions Materials References Publication
Extraction & Amplification of whole blood using WGA-Protocol
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This can hinder the researcher’s ability to perf...
GenomePlex Complete Whole Genome Amplification Kit
Product Description Components Storage Procedure References Troubleshooting Guide Frequently Asked Questions Disclaimer Automation Protocol Posters and Publications Reagents Required
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. Sigma-Aldrich offers many products for DNA extraction including the GenElute™ Blood Genomic DNA Kit (NA2010), GenElute Mamma...
Selecting Correctly Expressing Recombinants
Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation
SeqPlex Enhanced DNA Amplification Kit Protocol
Product Description Workflow Reagents Provided Storage/Stability Procedure Appendix 1: Optional Primer Removal Assay Troubleshooting Guide FAQs References
Keywords: AGE, Absorption, Amplification, Capillary electrophoresis, Chromatin immunoprecipitation, Digestions, Electrophoresis, Gel electrophoresis, Genomics, Immunoprecipitation, Microarray Analysis, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Reductions, Sequencing, Sonication, Substitutions, Whole genome amplification
SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms. Microgram quantit...
Soil DNA extraction & WGA Amplification - Protocol
Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be comp...
TransPlex® Whole Transcriptome Amplification Protocol
TransPlex, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3'-bias. Microgram quantities of ...
Keywords: Amplification, Centrifugation, Cloning, Condensations, DNA purification, Gene expression, Genomics, Microarray Analysis, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Sequencing
GenElute™ PCR Clean-Up Procedure [VIDEO]
The GenElute™ PCR Clean-up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as exc...
Keywords: Amplification, Cloning, DNA purification, Microarray Analysis, Polymerase chain reaction, Purification, Sequencing
GenomePlex® Amplification of ChIP DNA
ChIP-chip is a powerful tool that combines chromatin immunoprecipitation (ChIP) with microarrays to study protein-DNA interactions genome-wide. The ChIP step utilizes an antibody directed against a t...
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Gene expression, Immunoprecipitation, Methylations, Microarray Analysis, Nucleic acid hybridization, Sample preparations, Transcription, Whole genome amplification, transformation
PCR Selection Guide
Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative
Protein Tyrosine Kinase and Phosphatase Expression Profiling - Cancer Research
By focusing on analysis techniques and protocols specific for Cancer Research, Sigma-Aldrich® is putting your research needs first. We will be featuring different cancer research tools throughout the...
Keywords: Amplification, Antibiotics, Cancer, Catalysis, Cell signaling, Cloning, Digestions, Electrophoresis, Enzyme activity, Filtration, Gel electrophoresis, Gene expression, Molecular biology, Nucleic acid denaturation, Nucleic acid hybridization, Peptide synthesis, Polymerase chain reaction, Polymorphisms, Purification, Separation, Sequencing, Transcription, transformation