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NA2010 Sigma

GenElute Blood Genomic DNA Kit

sufficient for 70 purifications

Synonym: Blood Genomic DNA, Gen Elute

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Description

General description

The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Application

The purified genomic DNA is ready for downstream applications such as:
• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning

Features and Benefits

• Starting material: Up to 200 μl of fresh or aged blood
• Expected yield: Up to 10 μg
• Elution volume: 400 μl
• Time required: <40 min
• A260/A280 ratio: 1.6 - 1.9
• Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate

Principle

The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.

The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


D-Tube Dialyzers

Microcon Centrifugal Filters

All labs need water

Kit component also available separately

Description

Product #

Add to Cart

Column Preparation Solution SDS C2112
Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology SDS P2308
RNase A solution SDS R6148

Kit component only

Description

Product #

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Resuspension Solution SDS    
Lysis Solution C SDS    
Wash Solution Concentrate SDS    
Elution Solution (10 mM Tris-HCl, 0.5 mM EDTA, pH 9.0) SDS    
Nucleic Acid Binding Column SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Protocols

Blood Card - Extraction & Amplification WGA Protocol

Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream...

Experienced User Protocol GenElute™ Blood Genomic DNA Kit Protocol (NA2010, NA2020)

For experienced users, an abbreviated protocol is listed below.  For data, images, troubleshooting and other information, please see the full length protocol page.
Keywords: Digestions, PAGE

Extraction & Amplification of whole blood using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This can hinder the researcher’s ability to perf...

GenElute™ Blood Genomic DNA Kit Protocol (NA2010, NA2020)

Sigma’s GenElute™ Blood Genomic DNA Kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silic...
Keywords: AGE, Addition reactions, Amplification, Anticoagulants, Cell disruption, Centrifugation, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing

Plasma Serum DNA extraction & WGA Amplification Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples. One must use a robust DNA purification scheme capable of ...

Peer-Reviewed Papers
15

References

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