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NDEGLY Sigma

Native Protein Deglycosylation Kit

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Description

Application

The Native Protein Deglycosylation is designed for the deglycoslylation of N-linked oligosaccharides from PNGase F-resistant native proteins. Endoglycosidases F1, F2, and F3 are less sensitive to protein conformation than PNGase F and are more suitable for removal of all classes of N-linked oligosaccharides without protein denaturation.

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Amplified Detection

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Endoglycosidase F1 .3 U SDS    
Endoglycosidase F2 .1 U SDS    
Endoglycosidase F3 .1 U SDS    

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Endoglycosidase F1 reaction buffer 200 μL SDS    
Endoglycosidase F2 & 3 reaction buffer 200 μL SDS    
Safety & Documentation
Protocols & Articles

Articles

Deglycosylation Kits

GlycoProfile™ I Enzymatic In-gel N-Deglycosylation Kit GlycoProfile™ II Enzymatic In-solution N-Deglycosylation Kit Enzymatic Protein Deglycosylation Kit Native Protein Deglycosylation Kit
Glycobiology Analysis Manual, 2nd Edition
Keywords: Degradations, Digestions, Glycosylations, High performance liquid chromatography, Mass spectrometry, Methods, PAGE, Proteomics

Endoglycosidases

For some glycoproteins, no cleavage by PNGase F occurs unless the protein is denatured. For others, some or all of the oligosaccharides can be removed from the native protein after extensive incubati...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Buffers, Fucosylation, Type

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