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NXTRACT Sigma

CelLytic NuCLEAR Extraction Kit

For mammalian tissue or cultured cells

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.

Linkage

Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549

Application

NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.

General description

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amplified Detection

Ultrafree Centrifugal Filter Units

Kit component only

Description

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10× Lysis Buffer, Hypotonic 7 mL SDS    
3× Dilution and Equilibration Buffer 90 mL SDS    
1 M DTT .4 mL SDS    
Extraction Buffer 10 mL SDS    
IGEPAL® CA-630 10% Solution 4 mL SDS    
5× Lysis Buffer, Isotonic 14 mL SDS    

Standard component

Description

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Protease Inhibitor Cocktail 1 mL SDS P8340
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles

Articles

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
Mark Frei
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot

Peer-Reviewed Papers
15

References

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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