|Related Categories||Cell Biology, Cell Fractionation and Organelle Isolation, Cell Lysis Products, Cell Lysis and Protein Extraction, Cell Signaling and Neuroscience,|
|usage||1 kit sufficient for 10 extractions (1 ml packed cell volume)|
|1 kit sufficient for 100 extractions (100 μl packed cell volume)|
|shipped in||dry ice|
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A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. CelLytic NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.
Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
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|10× Lysis Buffer, Hypotonic 7 mL|
|3× Dilution and Equilibration Buffer 90 mL|
|1 M DTT 0.4 mL|
|Extraction Buffer 10 mL|
|IGEPAL® CA-630 10% Solution 4 mL|
|5× Lysis Buffer, Isotonic 14 mL|
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Certificate of Analysis
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot
Differential utilization of Ras signaling pathways by macrophage colony-stimulating factor (CSF) and granulocyte-macrophage CSF receptors during macrophage differentiation. Guidez, F., et al. Mol. Cell. Biol. 18, 3851-3861, (1998)
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