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O0132 Sigma

Anti-Orexin B antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Obesity Research, Antibodies to Hormones,
species reactivity   human, mouse, rat
application(s)   indirect ELISA: 1:8,000
  radioimmunoassay: 1:1,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HCRT(3060)
rat ... Hcrt(25723)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminus of human orexin B (amino acids 1-18 with C-terminally added lysine), conjugated to BSA. This sequence is highly conserved (single amino acid substitution) in mouse and rat orexin B and is different from the orexin A sequence.

General description

Orexin B also refers as hypocretin 2 is a 28 amino acid containing peptide, localized mainly in neurons in the lateral hypothalamic area. Anti-orexin B antibody can be used in indirect ELISA (diluted 1: 8,000) using human orexin B. Rabbit anti-orexin B antibody reacts specifically with orexin B from mouse, rat and human. The product does not cross react with mouse, rat and human orexin A, α-MSH, human leptin and neuropeptide Y.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-orexin B antibody can be used in radioimmunoassay (RIA) (diluted 1:1,000) for detecting orexin B.

Price and Availability


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