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O4886 Sigma

Monoclonal Anti-Opsin antibody produced in mouse

clone RET-P1, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, Neuroscience,
species reactivity   bovine, duck, amphibian, goldfish, rabbit, rat, turtle, tiger salamander, mouse, dove, quail, human
application(s)   electron microscopy: suitable
  immunocytochemistry: suitable using cultured cells
  immunohistochemistry (frozen sections): 1:10,000 using frozen sections of rat eye
  indirect ELISA: suitable
  radioimmunoassay: suitable
  western blot: suitable
clone   RET-P1, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 39 kDa by immunoblotting of rat retina (closely spaced doublet)
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... RHO(6010)
mouse ... Rho(212541)
rat ... Rho(24717)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rat retinal membranes.

General description

Mouse monoclonal clone RET-P1 anti-Opsin antibody recognizes an epitope located in amino acid residues 4-10 at the N-terminus of the rhodopsin molecule. In immunoblotting of rat retina, the antibody labels a closely spaced doublet of 39 kDa and less intense bands of 78 and 115 kDa, representing rhodopsin monomer and aggregates. Higher M.W. bands are stained in pituitary (251 and 288 kDa) and in hypothalamus (269 and 331 kDa) in addition to several lower M.W. bands. The product specifically labels the cell bodies, outer and inner segments (rods but not cones) of rat photoreceptor surface. It reacts with bovine, duck, amphibian, goldfish, rabbit, rat, turtle, tiger salamander, mouse, dove, quail, and human rhodopsin/opsin.

Photoreceptors are responsible for the initial step in visual processing, converting the signal of photon absorption into synaptic transmission. The visual pigment in vertebrate rods responsible for the absorption of light quanta is rhodopsin (also called opsin). Rhodopsin comprises >95% of the rod outer segments (ROS) intrinsic membrane protein and is a glycoprotein possessing two asparagine-linked oligosaccharide groups at amino acid residues 2 and 15 of the N-terminus. Light is absorbed by rhodopsin and the subsequent conformational change leads to the activation of a cyclic GMP phosphodiesterase through a specific G-protein intermediate, transducin.

Application

Mouse monoclonal clone RET-P1 anti-Opsin antibody is used to tag opsin/rhodopsin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunocytochemistry of cultured cells, immunohistochemistry (paraformaldehyde/glutaraldehyde-fixed, paraformaldehyde perfusion-fixed, frozen sections), immunoelectron microscopy, ELISA, competitive ELISA, and solid phase RIA. It is used as a probe to determine the presence and roles of opsin/rhodopsin in studies of rhodopsin location, functional properties and molecular mechanisms governing rod photoreceptor differentiation.

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