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P0083 Sigma

PRMT1 from rat

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous solution

Synonym: HMT1-like 2, HRMT1L2, Heterogenious nuclear ribonucleoprotein methyltransferase 1-like 2, IR1B4, Interferon receptor 1-bound protein 4, Protein arginine N-methyl transferase 1

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, DNA-RNA Transcription Regulators, Gene Regulation and Expression
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Prmt1(60421)

Description

Analysis Note

The N-methyltransferase activity is determined by detecting the level of radiolabel transfer from 3H-AdoMet (Methyl donor) to histone (Cat. No. H4380), which is arginine rich (methyl acceptor).

Unit Definition

The specific activity is ≥ 0.1 nmol/mgP/min. measured by 3H-AdoMet incorporation into histone for 30 minues at 30 °C.

Physical form

Solution of 50 mM Tris, pH 7.6, 5 mM DTT, 0.2% IGEPAL® CA-630, 150 mM NaCl, and 30% glycerol (w/v).

Biochem/physiol Actions

Methyl transferases catalyze the addition of methyl groups to nitrogen, carbon, sulfur, and oxygen atoms of small molecules, lipids, proteins, and nucleic acids. Eight mammalian protein arginine methyltransferases (PRMT) have been identified. PRMT1 is the predominant member of the methyl transferases, which catalyzes the protein arginine N-methylation reactions. PRMT1 is implicated in various cellular processes including: transcription, RNA processing, and signal transduction.

Legal Information

IGEPAL is a registered trademark of Rhodia Operations

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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