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P0134 Sigma

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain

lyophilized powder, ~0.2 units/mg protein

Synonym: 3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Signaling Enzymes,
form   lyophilized powder
mol wt   mol wt ~60 kDa
composition   Protein, ~80%
storage temp.   −20°C
Gene Information   cow ... PDE1A(281969)

Description

Analysis Note

Protein determined by Warburg-Christian unless specified otherwise.

Application

For assay of 3′,5′-cyclic nucleotides.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Preparation Note

Prepared as described by Butcher, R.W. and Sutherland, E.W., J. Biol. Chem., 237, 1244 (1962).

Quality

Contains inorganic pyrophosphatase and 5′-ATPase.
Substantially free of 5′-nucleotidase and 2′,3′-cyclic nucleotide phosphodiesterase.

Reconstitution

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. Both the activated and activator deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.

Unit Definition

One unit will hydrolyze 1.0μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH7.5 at 30°C.

Physical form

Lyophilized preparation containing near saturation levels of both protein activator and Ca++, and buffer salts as imidazole and magnesium sulfate

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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