• USA Home
  • P0294 - Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle

P0294 Sigma

Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle

For the Determination of ADP, buffered aqueous glycerol solution

Synonym: PK/LDH enzymes from rabbit muscle



Related Categories Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes, Enzymes, Inhibitors, and Substrates
form   buffered aqueous glycerol solution
concentration   600-1,000 units/mL pyruvate kinase
  900-1400 units/mL lactic dehydrogenase
storage temp.   −20°C


Unit Definition

Pyruvate kinase activity: One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.
Lactic dehydrogenase activity: One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Solution in 50% glycerol containing 10 mM HEPES, pH 7.0, 100 mM KCl and 0.1 mM EDTA

Biochem/physiol Actions

ADP Quantification Assay protocol for the use of PK/LDH in the determination of ADP. Solutions containing unkown concentrations of ADP can be substuted for reagent D in this protocol. Further dilutions of the ADP solution may be required

Lactate dehydrogenase from rabbit muscle can be inhibited by ascorbate. Aldolase and actin were shown to block this inhibitory effect.

Pyruvate kinase requires bivalent and monovalent cations such as Mg2+ and K+ respectively for activation to occur.

Price and Availability

Your Key to Success

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?