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P0520 Sigma

Phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart

lyophilized powder (contains imidazole buffer salts and magnesium sulfate), >0.1 units/mg protein (with added activator), PI fraction of Ho, H.C., et al., Biochim. Biophys. Acta, 429, 461 (1976).

Synonym: 3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine heart, PDE

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
form   lyophilized powder (contains imidazole buffer salts and magnesium sulfate)
mol wt   mol wt ~60 kDa
composition   Protein, ~75%
foreign activity   5′-ATPase, alkaline phosphatase, and 2′:3′-cyclic nucleotide phosphodiesterase <0.005 unit/mg protein
  5′-nucleotidase <0.01 unit/mg protein
  inorganic pyrophosphatase <0.5 unit/mg protein
storage temp.   −20°C
Gene Information   cow ... PDE1A(281969)

Description

Analysis Note

Protein determined by Warburg-Christian unless specified otherwise.

Packaging

Package size based on activated units

Preparation Note

Crude preparation which has been stripped of the protein activator, calmodulin.

Reconstitution

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. If stored at 4 °C, both the activated and activator deficient activity, may decrease approx. 30% in 24 hrs.

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Application

Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca2+. It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold.

For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

The herbicide Atrazine was shown inhibit phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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