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P0652 Sigma

Protease from Streptomyces griseus

Type XXI, ≥15 units/mg solid

Synonym: Actinase E, Pronase E, actinase E, pronase E

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteases, Proteases & Protein Sequencing,
type   Type XXI
form   powder
mol wt   mol wt ~50 kDa
storage temp.   −20°C

Description

Biochem/physiol Actions

Alkaline protease that is approximately twice as active at pH 11.0 than at the usual assay conditions for protease, pH 7.5 and 37 °C. By comparison, P5147 Type XIV protease is only approximately 25% as active at pH 11.0, 30 °C.

Packaging

500 units in glass bottle

Preparation Note

Collected from culture broth of S. griseus.

Specificity

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Unit Definition

One unit will hydrolyze casein to produce peptide equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 11.0 at 30 °C.

Application

Pronase E can be used to degrade antheraea pernyi silk fibroin films.

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.

This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.

This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein. Proteases from Streptomyces griseus and Streptomyces omiyaensis have been used in a study to identify residue 71 as a crucial residue for differences in topological specificities. Proteases have also been used in a study to investigate production of protease as a by-product of streptomycin production.

Physical properties

Completely inactivated by heating above 80 °C for 15-20 minutes.

Isoelectric point : 8.7
Inhibitors : Diisopropyl fluorophosphate, EDTA
Optimum pH : >=12
Optimum temperature : 60oC
pH Stability : pH 5.0 - 11.5 (25oC,24hr)
Thermal stability : below 50oC (pH 8.3, 15min)

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
UK9595000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Assay Procedure for Protease

The appearance of peptides is measured as tyrosine equivalent at 275nm by spectrophotomertry.
Keywords: Extinction coefficient

Peer-Reviewed Papers
15

References

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