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P0690 Sigma

Pectinase from Aspergillus niger

BioReagent, plant cell culture tested, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

Synonym: Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger

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Description

Application

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Pectinase P0690 is a natural pectinase (polygalacturonase) produced by Aspergillus niger. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Physical form

Solution in 40% glycerol

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay of Pectinase

3.2 Unit Definition = = One unit will liberate 1.0 micromole of galacturonic acid from poly- galacturonic acid per hour at pH 4.0 at 25ºC.
Keywords: Enzyme activity, Enzymology, Oxidations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

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