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P0899 Sigma

Poly-D-lysine hydrobromide

mol wt 70,000-150,000

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Properties

Related Categories Amino Acids, Biochemicals and Reagents, Homopolymers, Polyamino Acids, Polylysine and Polyornithine Attachment Factors More...
InChI Key   MEXAGTSTSPYCEP-UHFFFAOYSA-N
mol wt   mol wt 70,000-150,000
storage temp.   −20°C

Description

Components

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Analysis Note

This product has a molecular weight of 70,000-150,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Application

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Poly-D-lysine hydrobromide is used for the following applications:
• Used in animal cell culture
• Rat cortical neuron cultures
• Cell culture and siRNA treatment
• Primary cell cultures and adult neuronal network model
• Cortical astrocyte culture
• Immunofluorescence staining (HDF cells were plated onto poly-D lysine (P0899, Sigma-Aldrich) coated glass coverslips)
• NPC collection and culture conditions

Biochem/physiol Actions

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Packaging

1 g in poly bottle

50, 100, 500 mg in poly bottle

10 mg in glass bottle

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Poly(Amino Acid) Block Copolymers for Drug Delivery and other Biomedical Applications

Carmen Scholz Department of Chemistry, University of Alabama in Huntsville Huntsville, AL 35899 Email: scholzc@uah.edu
Keywords: Addition reactions, Asymmetric synthesis, Building blocks, Catalysis, Degradations, Living polymerization, PEGylations, Polymerization reactions, Precipitation, Proteomics, Ring-opening polymerization, Solvents

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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