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P1016 Sigma

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis

Grade I, buffered aqueous glycerol solution, 0.8-2.0 units/mg protein

Synonym: L-Phenylalanine ammonia-lyase, Phenylalanine Deaminase from Rhodotorula glutinis

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Properties

Related Categories 4.3.x.x C-N bonds, 4.x.x.x Lyases, Amino Acid Metabolism, Biochemicals and Reagents, Cell Biology,
type   Grade I
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C

Description

Analysis Note

Protein determined by biuret

Unit Definition

One unit will deaminate 1.0 μmole of L-phenylalanine to trans-cinnamate and NH3 per min at pH 8.5 at 30 °C.

Physical form

Solution in 60% glycerol, 3 mM Tris-HCl, pH 7.5, containing up to 0.5 M (NH4)2SO4

Application

Phenylalanine ammonia-lyase has been used in a study to assess the effect of heat treatment on lignification of postharvest bamboo shoots. It has also been used in a study to investigate the effect of light on gene expression and podophyllotoxin biosynthesis in Linum album cell culture.

Biochem/physiol Actions

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis can be cleaved and inactivated by the proteases chymotrypsin, subtilisin, and trypsin.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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