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P1067 Sigma

Pullulanase from Klebsiella pneumoniae

lyophilized powder, 10-30 units/mg protein

Synonym: Amylopectin 6-gluconohydrolase, Limit dextrinase

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Description

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25 °C.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer

Application

Pullulanase has been used in a study to assess its location in Escherichia coli K12 carrying the cloned structural gene from Klebsiella pneumoniae. It has also been used in a study to investigate the role of lipid modification on the enzyme in comparison to unmodified pullulanases.

General description

Pullulanase is a lipoprotein generated as a precursor containing a 19-amino acid signal peptide followed by a palmitate-modified cysteine residue. The signal peptide gets cleaved prior to secretion into the extracellular matrix.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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