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P1261 Sigma

Phosphorylase a from rabbit muscle

lyophilized powder, 20-30 units/mg protein

Synonym: 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase

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Properties

Related Categories 2.4.x.x Glycosyltransferases, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
purified by   2× crystallization
composition   Protein, 30-60% E1%/280
foreign activity   AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%
  phosphoglucomutase ≤0.25%
  phosphorylase b ≤30%
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Frequently Asked Questions are available for this Product.

General description

Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).

Packaging

10, 25 mg in poly bottle

Unit Definition

One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)

Physical form

Lyophilized powder containing β-glycerophosphate and EDTA

Application

Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of 32P-ATP.

Biochem/physiol Actions

Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.

Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How is Phosphorylase A, Product P1261, prepared?
To prepare Phosphorylase A (P1261), Sigma uses a method similar to the one described in the J. Biol.Chem., 231, 65 (1958).
What is the final buffer Phosphorylase A, Product P1261, is in prior to lyophilization?
The final buffer Phosphorylase A (P1261) is in prior to lyophilization is 0.1M B-glycerophosphate and 4 mM EDTA.        
What is the solubility of Phosphorylase A, Product P1261?
Sigma tests solubility of Phosphorylase A (P1261) in water at a concentration of 1 mg/ml.   
What is the molecular weight of Phosphorylase A, Product P1261?
The molecular weight of Rabbit    Phosphorylase A (tetramer) is 370,000 per Biochemistry 6 (1967) p. 3315 (which was determined by sedimentation and "experiments in a variety of buffers, temperatures, and ionic strenght").  The molecular weight by sequence analysis (Proc. Natl. Acad. Sci. USA, 74, 4762-4766) is 97,412.             
What is the pI of Phosphorylase A, Product P1261?
The pI of Phosphorylase A (P1261) is 5.6 - 5.8.
Has the amino acid sequence of phosphorylase from rabbit muscle been published?
The sequence has been published. The amino acid composition is: Asp5l, Asn45, Thr35, Ser29, Glu64, Gln31, Pro36, Gly48, Ala63, Cys9, Val62, Met2l, Ile49, Leu79, Tyr36, Phe38, Trp12, Lys48, HiS22, and Arg63. Reference for the sequence and amino acid composition: K. Titani et al., Proc. Nat. Acad. Sci., USA, 74, 4762-4766 (1977) (see attached pdf). Product P1261 is phosphorylase a, which is the tetrameric form. Products P4649 and P6635 are phosphorylase b, the dimeric  form. The sequence information of the subunit would be the same for both forms.
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Protocols & Articles

Articles

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

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Peer-Reviewed Papers
15

References

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