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P1274 Sigma

Poly-L-lysine hydrobromide

mol wt 70,000-150,000 by viscosity

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Properties

Related Categories Amino Acids, Biochemicals and Reagents, Homopolymers, Polyamino Acids, Polylysine and Polyornithine Attachment Factors More...
Quality Level   PREMIUM
form   solid
mol wt   mol wt 70,000-150,000 by viscosity
storage temp.   −20°C

Description

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Analysis Note

Molecular weight based on viscosity and is also assayed by MALLS.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Packaging

1 g in poly bottle

25, 100, 500 mg in poly bottle

Preparation Note

This product has a molecular weight of 70,000-150,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Poly-L-lysine hydrobromide is used for the following applications:
• Used for some experiments with REF-52 cells, for coating
• For electrophysiological experiments, cells were seeded on poly-L-lysine hydrobromide coated or uncoated cover-slips
• Cell culture of rat neurons
• Indirect binding of monoclonal antibody via poly-llysine

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Poly(Amino Acid) Block Copolymers for Drug Delivery and other Biomedical Applications

Carmen Scholz Department of Chemistry, University of Alabama in Huntsville Huntsville, AL 35899 Email: scholzc@uah.edu
Keywords: Addition reactions, Asymmetric synthesis, Building blocks, Catalysis, Degradations, Living polymerization, PEGylations, Polymerization reactions, Precipitation, Proteomics, Ring-opening polymerization, Solvents

Related Content

Bio Brief: Poly-L-lysine Hydrobromide [VIDEO]

Poly-L-lysine hydrobromide is a non-specific attachment factor useful for promoting cell adhesion to solid substrates, such as coverslips, slides, and microwell plates. Poly-L-lysine is a positively-...
Keywords: Adhesion

Peer-Reviewed Papers
15

References

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