Synonym: PAP
MDL number MFCD00151734
Popular Documents: Datasheet (PDF)
| Related Categories | Alphabetical Index, Antibodies, PAP, PD-PG, Primary Antibodies, |
| antibody form | affinity isolated antibody |
| form | buffered aqueous solution |
| application(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 |
| shipped in | dry ice |
| storage temp. | −20°C |
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal
Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.
The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.
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