P1291 Sigma

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym: PAP

Properties

Related Categories Alphabetical Index, Antibodies, PAP, PD-PG, Primary Antibodies,
antibody form   affinity isolated antibody
form   buffered aqueous solution
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
shipped in   dry ice
storage temp.   −20°C

Description

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.

Biochem/physiol Actions

The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.

Price and Availability

Anti-Rabbit 2° Antibody Best Sellers


Prod. No.ConjugateSecondary Antibody Description
A0545HRPAnti-Rabbit IgG (whole molecule)-Peroxidase
A3687APAnti-Rabbit IgG (whole molecule)-Alkaline Phosphatase
F0382FITCAnti-Rabbit IgG (whole molecule)-FITC
B8895BiotinAnti-Rabbit IgG (whole molecule)-Biotin





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