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P1364 Sigma

Purification and Analysis of Recombinant Proteins

  •  ISBN-10 0-8247-8277-1

  •  ISBN-13 978-0-8247-8277-1

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publication info   R. Seetharam and S. K. Sharma, Marcel Dekker, 1991, 344 pp., hard cover
mfr. no.   Marcel Dekker

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General description

Covering vital and traditional topics in the purification and analysis of recombinant proteins, this volume demonstrates how to overcome problems in protein research and presents practical methods used in protein work, explaining their theoretical basis. The collection also explores innovative concepts, such as receptor affinity chromatography and the introduction of sites that facilitate purification. This aid uses specific examples of proteins obtained from bacterial and nonbacterial sources and supplies helpful approaches and techniques.

Table of Contents

I.INTRODUCTION
1. Purification and Characterization of Recombinant Proteins: Opportunities and Challenges
2. Purification and Production of Therapeutic Grade Proteins
II. TECHNICAL ISSUES RELATED TO RECOVERY OF RECOMBINANT PROTEINS
3. Physical and Chemical Cell Disruption for the Recovery of Intracellular Proteins
4. Proteases During Purification
5. Properties of Recombinant Protein-Containing Inclusion Bodies in Escherichia Coli
6. Methods for Removing N-Terminal Methionine from Recombinant Proteins
III. PURIFICATION OF RECOMBINANT PROTEINS FROM Escherichia Coli, YEAST, AND MAMMALIAN CELLS
7. Purification of Secreted Recombinant Proteins from Escherichia Coli
8. Purification of Recombinant Proteins from Yeast
9. Production of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
10. Purification of Monoclonal Antibodies
IV. RECENT TRENDS IN THE AREA OF RECOMBINANT PROTEIN PURIFICATION AND ANALYSES
11. Engineering Proteins to Enable Their Isolation in a Biologically Active Form
12. Practical Aspects of Receptor Affinity Chromatography
13. Recombinant DNA Technology and Crystallography: A New Alliance in Unraveling Protein Structure-Function Relationships

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