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P1473 Sigma

PCR 100 bp Low Ladder

for electrophoresis of PCR fragments

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Properties

Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
grade   for molecular biology
form   liquid
usage   75 uses
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

Components

Sigma’s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

General description

Sigma’s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Other Notes

Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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Description

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D7808 PCR Low Ladder Set, marker set for electrophoresis of PCR fragments
D3687 DirectLoad PCR 100 bp Low Ladder, ready-to-use marker for DNA electrophoresis
P1598 PCR 20 bp Low Ladder, for electrophoresis of PCR fragments
P9577 PCR 50 - 2,000 bp Marker, for electrophoresis of PCR fragments

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