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Protein Phosphatase Protocols: Methods in Molecular Biology, Vol. 93

  •  ISBN-10 0-89603-468-2

  •  ISBN-13 978-0-89603-468-6

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publication info   J. Ludlow, ed., Humana Press, 1998, 336 pp., hard cover
mfr. no.   Humana

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General description

Cutting-edge techniques presented with step-by-step instructions make this indispensable for investigating the structure and function of protein serine/threonine phosphatases. Contains methods designed to isolate, identify, and assay phosphatase activities from both prokaryotic and eukaryotic sources. Some of the points covered are step-by-step instructions on how to isolate novel phosphatases and regulatory subunits; detailed methods for generating immunological reagents to characterize protein phosphatases; cloning of novel phosphatases and regulatory subunits; notes about potential pitfalls and tips on how to avoid failure.

Table of Contents

Prokaryotic Protein-Serine/Threonine Phosphatases,
Protein Phosphatase Type 1 and 2A Assays,
Analyzing Gene Expression with the Use of Serine/Threonine Phosphatase Inhibitors,
Inhibitor-1, a Regulator of Protein Phosphatase-1 Function.
I1PP2A and I2PP2A: Two Potent Protein Phosphatase 2A-Specific Inhibitor Proteins,
Control of PP1 Activity Through Phosphorylation by Cyclin-Dependent Kinases,
Regulation of Neuronal PP1 and PP2A During Development.
PTPA Regulating PP2A as a Dual Specificity Phosphatase.
Microinjection and Immunological Methods in the Analysis of Type 1 and Type 2A Protein Phosphatases from Mammalian Cells,
Use of Immunocomplexed Substrate for Detecting PP1 Activity,
The Biochemical Identification and Characterization of New Species of Protein Phosphatase 1.
The Relationship between insulin signaling and protein phosphatase 1 activation,
Analysis of the isoforms of protein phosphatase (PP1) isoforms with polyclonal peptide antibodies,
Expression of mouse protein phosphatase 2C in Escherichia coli and COS 7 cells,
Expression of Functional protein phosphatase 1 catalytic subunit in Escherichia coli,
Protein Phosphatase 2A and Protein Phosphatase X Genes in Arabidopsis thaliana.
Separation of Protein Phosphatase Type 2C Isozymes by Chromatography on Blue Sepharose,
Chromatographic Isolation of PP2A from Limulus Lateral Eyes: Conventional and Small Scale Methods,
Purification and Assay of the Ptc/Tpd1 Protein Phosphatase 2C from the yeast Saccharomyces cerevisiae.
Molecular cloning of protein phosphatase type 2C isoforms from retinal cDNA.
Analysis of Protein Interactions Between Protein Phosphatase 1 and Noncatalytic Subunits using the Yeast Two-Hybrid Assay.
Identifying Protein Phosphatase 2A Interacting Proteins Using the Yeast Two-hybrid method,
Protein phosphatase 2A regulatory subunits: cDNA cloning and analysis of mRNA expression, Synthetic Lethal Screening in Protein Phosphatase Pathways .
The Search for the Biological Function of Novel Yeast ser/thr Phosphatases,
Index.

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