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P1484 Sigma

Monoclonal Anti-p21WAF1/Cip1 antibody produced in mouse

clone CP74, purified from hybridoma cell culture

Synonym: Anti-CDKN1A, Anti-Cip1, Anti-WAF1, Anti-p21

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human, mouse
application(s)   immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 5-10 μg/mL using LNCap cells (human prostate adenocarcinoma) treated with MG132 proteosome inhibition.
clone   CP74, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
mol wt   antigen mol wt 21 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CDKN1A(1026)
mouse ... Cdkn1a(12575)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human p21WAF1/Cip1.

General description

Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody reacts specifically with p21WAF1/Cip1. It shows no cross-reactivity with other closely related mitotic inhibitors. The epitope recognized by the antibody resides in the N-terminal amino acids 17-30 within the cyclin-kinase binding domain of the p21WAF1/Cip1 molecule. Reactivity has been observed with human and mouse p21WAF1/Cip1.

Regulation of cell cycle progression in eukaryotic cells depends on the expression of proteins called cyclins. These proteins form complexes with several different cyclin dependent kinases (CDKs). The catalytic activity of cyclin dependent kinases (CDK) is regulated by two general mechanisms: protein phosphorylation and association with regulatory subunits, which include the cyclins and the CDK inhibitors (CKIs). Two families of CKIs have been identified. The p21WAF1/Cip1 family contains p21WAF1/Cip1, p27Kip1 and p57Kip2. These inhibit all kinases involved in the G1/S transition. The the p16INK4a family, which includes p15INK4b, p16INK4a, p18INK4c and p19INK4d, inhibits Cdk4 and Cdk6 specifically.

Physical form

Solution in phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide

Application

Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody may be used for ELISA, immunoblotting (21 kDa) and immunoprecipitation (p21/cyclin A/Cdk2 complexes, native and denatured). The availability of a monoclonal antibody reacting specifically with p21WAF1/Cip1 enables the subcellular detection and localization of p21WAF1/Cip1 and the measurement of relative differences in p21WAF1/Cip1 levels as a function of cell cycle phase.

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