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P1526 Sigma

o-Phenylenediamine dihydrochloride

peroxidase substrate

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Properties

Related Categories Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Detection, Detection Substrates,
Quality Level   PREMIUM
InChI Key   RIIWUGSYXOBDMC-UHFFFAOYSA-N
color   white to off-white
mp   258 °C (dec.)(lit.)
storage temp.   −20°C

Description

Caution

May darken in storage.

Packaging

1 kg in glass bottle

10, 25, 50, 100 g in glass bottle

Application

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1673 6.1 / PGIII
WGK Germany 
3
RTECS 
ST0175000
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

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Peer-Reviewed Papers
15

References

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