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P1601 Sigma

Anti-Protein Kinase Bα antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Akt1, Anti-PKBα

Properties

Related Categories AKT Antibodies, AKT1 Antibodies, Alphabetical Index, Antibodies, Antibodies for Apoptosis,
species reactivity   mouse, rat, bovine, human
application(s)   microarray: suitable
  western blot: 1:5,000 using human MCF7 cell extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 56 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Synthetic peptide corresponding to amino acids 462-477 of human PKBα/Akt1 conjugated to BSA.

General description

Protein Kinase B (also known as Akt, or RAC-PK) is a protein that belongs to serine/threonine kinase familywhich regulates many functions like cell proliferation, differentiation, cell cycle and apoptosis. It also has a pivotal role in protein and glycogen synthesis. Anti-protein kinase B α antibody can be used for studying the expression and function of PKB/Akt isoforms in different cells and tissues. These antibodies can also be used to correlate the expression pattern of PKB/Akt isoforms with pathological conditions or physiological functions. Rabbit anti-protein kinase B α antibody reacts specifically with PKB α.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-protein kinase B α antibody can be used in immunoblotting to detect and localize PKB α/Akt1using whole cell extract of MCF7 cultured cells. It can also be used in microarray.

Price and Availability


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