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P1644 Sigma

Percoll®

pH 8.5-9.5 (20 °C)

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.

Legal Information

Percoll is a registered trademark of GE Healthcare

Price and Availability


Tissue Diagnostics

Ultrafree Centrifugal Filter Units

Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Can you help me calculate gradients for Percoll®, Product P1644?
The Product Information Sheet has an equation for calculating density of a given solution:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/p1644pis.Par.0001.File.tmp/p1644pis.pdf
When using Product P1644, Percoll®, what density should I use for my particular system?
The Product Information Sheet has a table of densities for different organelles and cell types: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/p1644pis.Par.0001.File.tmp/p1644pis.pdf
Is there a "best" salt solution to use with Product P1644, Percoll®?
Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested.  Sucrose is included to prevent aggregation.  You can find detailed preparation instructions on the product information sheet for this product:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/p1644pis.Par.0001.File.tmp/p1644pis.pdf
Can Percoll®, Product P1644, be autoclaved?
Percoll can be autoclaved, but this must be done without any salts or sucrose present.  This, along with other useful information, can be found on the product information sheet:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/p1644pis.Par.0001.File.tmp/p1644pis.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the difference between Percoll® Products P4937 and P1644?
Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.
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Protocols & Articles
Peer-Reviewed Papers
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References

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