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P1644 Sigma

Percoll®

pH 8.5-9.5 (25 °C)

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Description

Frequently Asked Questions

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Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses.1 It has been used to separate inner and outer membrane vesicles from E. coli.2 Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.

Legal Information

Percoll is a registered trademark of GE Healthcare

Price and Availability

TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
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Safety & Documentation

Safety Information

Personal Protective Equipment 
WGK Germany 
3

Protocols & Articles

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll). H. Pertoft et al. Anal. Biochem. 88, 271, (1978)

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2. Separation of inner and outer membrane vesicles from Escherichia coli in self-generating Percoll gradients. S. Morein et al. Anal. Biochem. 216, 47, (1994)

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Improved methods to isolate and subfractionate rat liver mitochondria. Lipid composition of the inner and outer membrane. Hovius, R., et al. Biochim. Biophys. Acta 1021, 217-226, (1990)

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A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase. Hirano, T., et al. J. Cell Biol. 106, 1171-1183, (1988)

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Medulloblastoma can be initiated by deletion of Patched in lineage-restricted progenitors or stem cells. Yang ZJ Cancer Cell 14(2), 135-45, (2008)

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The Wiskott-Aldrich syndrome protein is required for iNKT cell maturation and function. Locci M J. Exp. Med. 206(4), 735-42, (2009)

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Platelet factor 4 mediates inflammation in experimental cerebral malaria. Srivastava K Cell Host Microbe 4(2), 179-87, (2008)

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Airborne lipid antigens mobilize resident intravascular NKT cells to induce allergic airway inflammation. Scanlon ST, Thomas SY, Ferreira CM, et al. J. Exp. Med. 208(10), 2113-24, (2011)

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BDNF-mediated cerebellar granule cell development is impaired in mice null for CaMKK2 or CaMKIV. Kokubo M J. Neurosci. 29(28), 8901-13, (2009)

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Effects of long-term starvation on a host bivalve (Codakia orbicularis, Lucinidae) and its symbiont population. Audrey Caro et al Appl. Environ. Microbiol. 75, 3304-13, (2009)

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Morphological, molecular, and phylogenetic characterization of Nosema ceranae, a microsporidian parasite isolated from the European honey bee, Apis mellifera. Chen YP J. Eukaryot. Microbiol. 56(2), 142-7, (2009)

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A role for VAMP8/endobrevin in surface deployment of the water channel aquaporin 2. Wang, C.C., et al. Mol. Cancer Ther. 30, 333-43, (2010)

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Ets-2 and p53 mediate cAMP-induced MMP-2 expression, activity and trophoblast invasion. Staun-Ram E Reprod. Biol. Endocrinol. 7, 135, (2009)

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Durable mucosal simian immunodeficiency virus-specific effector memory T lymphocyte responses elicited by recombinant adenovirus vectors in rhesus monkeys. Li H, Liu J, Carville A, et al. J. Virol. 85(21), 11007-15, (2011)

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CD36 deletion improves recovery from spinal cord injury. Myers SA, Andres KR, Hagg T, et al. Exp. Neurol. 256, 25-38, (2014)

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Astrocytic TGF-β signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection. Cekanaviciute E, Dietrich HK, Axtell RC, et al. J. Immunol. 193(1), 139-49, (2014)

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Niaspan treatment increases tumor necrosis factor-alpha-converting enzyme and promotes arteriogenesis after stroke. Chen J, Cui X, Zacharek A, et al. J. Cereb. Blood Flow Metab. 29(5), 911-20, (2009)

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A microfluidic technique for monitoring bloodstream analytes indicative of C-peptide resistance in type 2 diabetes. Teresa D’Amico Oblak et al Analyst 134, 188-93, (2008)

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Erythrocyte scaffolding protein p55/MPP1 functions as an essential regulator of neutrophil polarity. Quinn BJ Proc. Natl. Acad. Sci. U. S. A. 106(47), 19842-7, (2009)

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Sequential priming with simian immunodeficiency virus (SIV) DNA vaccines, with or without encoded cytokines, and a replicating adenovirus-SIV recombinant followed by protein boosting does not control a pathogenic SIVmac251 mucosal challenge. Thorsten Demberg et al J. Virol. 82, 10911-21, (2008)

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Mice with an absent stress response are protected against ischemic renal injury. Sreedharan R, Chen S, Miller M, et al. Kidney Int. 86(3), 515-24, (2014)

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The dendritic and T cell responses to herpes simplex virus-1 are modulated by dietary vitamin E. Sheridan PA Free Radic. Biol. Med. 46(12), 1581-8, (2009)

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Protection from experimental cerebral malaria with a single dose of radiation-attenuated, blood-stage Plasmodium berghei parasites. Gerald NJ, Majam V, Mahajan B, et al. PLoS ONE 6(9), e24398, (2011)

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Pathogenic MOG-reactive CD8+ T cells require MOG-reactive CD4+ T cells for sustained CNS inflammation during chronic EAE. Bettini M J. Neuroimmunol. 213(1-2), 60-8, (2009)

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Nerve growth factor regulates synaptophysin expression in developing trigeminal ganglion neurons in vitro. Tarsa L Neuropeptides 43(1), 47-52, (2009)

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HAC stability in murine cells is influenced by nuclear localization and chromatin organization. Moralli, D BMC Cell Biol. 10, 18, (2009)

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Douce, R. and Joyard, J., Edelman, M., Hallick, R., and Chua, N.H. Methods in Chloroplast Molecular Biology Amsterdam , (1982), 239-256

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