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P1709 Sigma

Peroxidase, Maleimide Activated from horseradish

lyophilized powder, >200 units/mg protein

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Properties

Related Categories Activated HRP for Conjugation, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents, Chemically Modified HRP,
form   lyophilized powder
composition   Protein, ~50% E1%/403
extent of labeling   1-3 mol maleimide per mol protein
storage temp.   −20°C

Description

Other Notes

Type VI peroxidase activated with maleimidocaproic acid N-hydroxysuccinimide ester.

Physical form

Lyophilized powder containing trehalose and sodium citrate

Application

For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent.
Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase.

To increase signal intensity of immunoconjugates, immunoglobulins were synthetically modified to contain multiple primary amines and covalently coupled to maleimide activated peroxidase. This can result in more efficient conjugation and signal amplification.

Price and Availability

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate. Products using this method include, but are not limited to, P1709, P2088, P6140, P6782, P81...
Keywords: Enzyme activity, Extinction coefficient

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275). This ratio (A403/A275) is not a measure of enzyme activity. Products using this me...
Keywords: Enzyme activity

Peer-Reviewed Papers
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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