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P1790 Sigma

Phosphodiesterase, 3′,5′-Cyclic Nucleotide, Activator-deficient from porcine brain

lyophilized powder

Synonym: 3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from porcine brain, PDE

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Signaling Enzymes,
form   lyophilized powder
activity   1.0-5.0 units/mg protein (without added calmodulin.)
  15-30 units/mg protein (in the presence of 0.03 mM Ca2+ and a saturating level - 10 units per ml - of calmodulin, P2277)
mol wt   mol wt ~60 kDa
purified by   affinity chromatography
contains   lactose as stabilizer
composition   Protein, ~5% Lowry
foreign activity   5′-ATPase, inorganic pyrophosphatase, alkaline phosphatase and 2′,3′-cyclic nucleotide phosphodiesterase <0.1%
  5′-nucleotidase <0.5%
storage temp.   −20°C
Gene Information   pig ... PDE3A(396555)

Description

Analysis Note

Protein determined by Warburg-Christian unless specified otherwise.

Application

May be used to assay the protein activator, calmodulin.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Packaging

Package size based on activated units.

Reconstitution

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Physical form

Lyophilized preparation which has been depleted of calmodulin and containing buffer salts as Tris-HCl.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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