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P1863 Sigma

PARP-1 human

buffered aqueous glycerol solution, recombinant, expressed in Sf9 cells, >98% (Purified by affinity chromatography)

Synonym: NAD+ ADP-ribosyltransferase, Poly(ADP-ribose) Polymerase

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, PARP (Poly(ADP-Ribose) polymerase), PARP (Poly(ADP-Ribose) polymerase) Enzymes More...
recombinant   expressed in Sf9 cells
assay   >98% (Purified by affinity chromatography)
form   buffered aqueous glycerol solution
activity   ≥500 units/mg protein
storage temp.   −70°C
Gene Information   human ... PARP1(142)

Description

Unit Definition

One unit synthesizes 1nmol of poly(ADP-ribose) per min at pH 7.5 at 25°C.

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, containing 14 mM β-mercaptoethanol, 0.5 mM PMSF, and 10% glycerol.

Biochem/physiol Actions

PARP-1, a nuclear enzyme that synthesizes ADP-ribose polymers from NAD, specifically binds Zn2+ and DNA, and recognizes single-strand breaks in DNA. It is involved in base excision repair, both short-patch and long-patch, rejoining DNA strand breaks and plays a role in p53 expression and activation. A high level of basal neuronal DNA damage and PARP activity has been reported in rat brain tissue. PARP-1 was shown to be required for HIV-1 integration into DNA. If PARP-1 is deficient there is no productive HIV-1 infection.

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Flow Cytometry Instruments
Safety & Documentation

Safety Information

WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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