• USA Home
  • P1867 - Plasmin from human plasma

EMAIL THIS PAGE TO A FRIEND
P1867 Sigma

Plasmin from human plasma

lyophilized powder, ≥2.0 units/mg protein

Synonym: Fibrinolysin

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Plasmin functions as a key enzyme of the fibrinolytic cascade, and is also important in inflammation processes.

Packaging

Package size based on protein content

Unit Definition

One unit will produce one μmole of p-Nitroanilide from D-Val-Leu-Lys-p-Nitroanilide per minute at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing sodium phosphate, mannitol, and NaCl.

Application

A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin.

Biochem/physiol Actions

Plasmin exhibits preferential cleavage at the carboxyl side of lysine and arginine residues with higher selectivity than trypsin. Converts polymerized fibrin into soluble products.
pH Optimum: 8.5
pH 7.5: ~40% of maximal activity, pH 9.5: ~50% of maximal activity
Temperature Optimum: 37 °C with rapid inactivation at 56 °C

Plasmin is inhibited by α 2-antiplasmin which binds to plasmin blocking its interaction with fibrin. Plasmin digests the N-terminal region of the cytoplasmic protein αsynuclein preventing its uptake by surrounding cells.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
Can Product P1867, Plasmin from human plasma, be stored?
Sigma has not evaluated the solution stability of plasmin solutions. It is best to prepare solutions fresh.
Does Product P1867, Plasmin from human plasma, have a recommended retest date?
If a product has a recommended retest date this would be on the Certificate of Analysis found at our website.  If no recommended retest date is found on the Certificate of Analysis, then the guarantee of quality is one year from the time of delivery for unopened bottles stored at the recommended temperature. See general information on product dating in the following link to our website: http://www.sigmaaldrich.com/sigma-aldrich/general_information/ProductDatingandShipping.pdf
Is there a conversion to International Units (I.U.) for Product P1867, Plasmin from human plasma?
Sigma has not evaluated the activity of this enzyme in I.U., nor do we have a conversion factor.
What is the molecular weight of Product P1867, Plasmin from human plasma?
The molecular weight of this dimeric protein was reported to be 75,400 Daltons. Reference is: The Plasma Proteins, Volume 1, 2nd ed., Putnam, F.W., ed., p. 66 (1975).
What is Product P1867, Plasmin from human plasma, soluble in?
It is soluble in water.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Can you provide a method of production for Product P1867 - Plasmin?
Product P1867 - Plasmin from human plasma is from an outside supplier and the manufacturing process is considered proprietary.  The product is activated with Streptokinase and this enzyme is removed in the final steps of the purification.  The product is >95% by SDS PAGE. The product is >3% protein (the exact value will be on the lot specific Certificate of Analysis).  This material is lyophilized from 20 mM sodium phosphate (pH 7.4), containing 10 mg/ml D-mannitol and 10 mg/ml NaCl.  The lyophyllization buffer will contribute a significant amount of solids to the product.  The product package size is based on protein content.
Show more questions
Protocols & Articles

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Protocols

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

This scope of this procedure applies to products that have a specification for the enzymatic activity of plasmin.
Keywords: Enzyme activity, Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?