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  • P1874 - Monoclonal Anti-Polyglutamines antibody produced in mouse

P1874 Sigma

Monoclonal Anti-Polyglutamines antibody produced in mouse

~2 mg/mL, clone 3B5H10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Huntingtin



Related Categories Alphabetical Index, Antibodies, Antibodies for Huntington’s Disease Research, Antibodies to Transcription Factors, Cell Biology,
species reactivity   human
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  western blot: 1-2 μg/mL using extract of HEK-293T cells transfected with an N-terminal 171 amino acid fragment of human Huntingtin with a 68 glutamine stretch
clone   3B5H10, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HTT(3064)
biological source   mouse
conjugate   unconjugated



GST-human Huntingtin (N-terminal fragment of 171 amino acids containing 65Q).

General description

Abnormal stretch of triplet CAG (or CAA) codon produces a homomeric repeat of glutamine residues beyond the critical threshold and will lead to neurodegenerative disease. Monoclonal mouse anti-polyglutamine antibody can be used to detect poly Q protein expression and in western blot. Monoclonal anti-polyglutamines antibody reacts specifically to homomeric polyglutamines independent of the species.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal anti-polyglutamines antibody may be used for ELISA, immunoblot and slot blot. It can also be used in immunoprecipitation and immunocytochemistry.

Legal Information

This product is for in vitro use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California. This product is sold under license to U.S. Patent No. 6,291,652, owned by the Regents of the University of California.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Huntington's Disease

Huntington's disease (HD) is an autosomal dominant, late-onset neurodegenerative disorder characterized by a selective neuronal cell death in the cortex and striatum leading to cognitive dysfunction,...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 9–14
Keywords: Apoptosis, Huntington Disease, Transcription

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Peer-Reviewed Papers


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06693 Timestrip Plus -20 °C

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