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P1901 Sigma

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym: PAP

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Properties

Related Categories Alphabetical Index, Antibodies, PAP, PD-PG, Primary Antibodies,
biological source   goat
antibody form   affinity isolated antibody
form   buffered aqueous solution
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

1 mL in glass bottle

Physical form

Solution in 8 mM sodium acetate, 15 mM ammonium acetate buffered saline, pH 7.2, containing 0.5% bovine serum albumin and 15 mM sodium azide.

Application

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in goat was used for immunohistochemistry of frozen brain sections of adult monkeys and porcine kidneys at a dilution of 1:500.

Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.

Biochem/physiol Actions

The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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