• USA Home
  • P1951 - Phalloidin–Tetramethylrhodamine B isothiocyanate

P1951 Sigma

Phalloidin–Tetramethylrhodamine B isothiocyanate

Phalloidin from Amanita phalloides

Synonym: Phalloidin-TRITC



Biochem/physiol Actions

Toxin that binds polymeric F actin, stabilizing it and interfering with the function of actin-rich structures.

Phalloidin interacts with polymeric actin, and not oligomeric or monomeric forms. This interaction leads to highly stabilized actin filaments, which resist depolymerization and disassembly. In rats, this toxin causes death due to liver hemorrhage, and cells show abnormal actin clustering. The affinity of phalloidin to actin is not significantly altered after derivatizing florescent labelled phalloidin compounds. These compounds can be used to study actin structure and organization within eukaryotic cells.

Other Notes

Mixed isomers


Fluorescent phallotoxin which may be used to identify filamentous actin.

Phalloidin-Tetramethylrhodamine B isothiocyanate has been used:-
• In Immunofluorescence for staining Filamentous actin (F-actin)
• To stain cells during immunocytochemical and cytochemical analysis
• To label actin microfilaments for fluorescence microscopy

General description

Phalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide, which interacts with actin, and this was first identified in phalloidin-poisoned rats. It is a heptapeptide, cyclic in nature, with a crosslink between tryptophan at position 6 and cysteine at position 3. The side chain of amino acid 7 (γ-δ-dihydroxyleucine) in phalloidin, is accessible to modifications, through which florescent labelled phalloidin compounds can be produced.

Price and Availability

All labs need water
Safety & Documentation

Safety Information

GHS06  GHS06
Signal word 
Hazard statements 
Precautionary statements 
UN 3462 6.1 / PGII
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Hematopoietic Cytokines

Numerous cytokines are involved in the regulation of hematopoiesis within a complex network of positive and negative regulators. Some cytokines have very narrow lineage specificities of their actions...
Jennifer Fries
BioFiles 2009, 4.5, 8.
Keywords: Applications, Clinical, Growth factors, Ligands

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?