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P2023 Sigma

Phospho(enol)pyruvate Carboxylase from corn

ammonium sulfate suspension, ≥1 units/mg protein

Synonym: Orthophosphate:oxaloacetate carboxylyase-(phosphorylating), PEP-carboxylase, Phosphoenolpyruvate Carboxylase from maize leaves

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Properties

Related Categories 4.1.x.x C-C bonds, 4.x.x.x Lyases, Application Index, Biochemicals and Reagents, Cell Biology,
form   ammonium sulfate suspension
foreign activity   malic dehydrogenase, variable
  malic enzyme, variable
storage temp.   2-8°C

Description

General description

Phospho(enol)pyruvate carboxylase is a ubiquitous, highly regulated oligomeric, cytosolic enzyme in plants.

Unit Definition

One unit will form 1.0 μmole of oxaloacetate from phospho(enol)pyruvate and CO2 per min at pH 8.5 at 25 °C.

Physical form

Suspension in 2.4 M (NH4)2SO4 solution containing 10 mM phosphate buffer, pH 7.0, 1 mM biotin, 5 mM dithiothreitol and 1 mM phenylmethylsulfonyl fluoride

Application

Phospho(enol)pyruvate carboxylase has been used in a study to assess activity of carbon metabolism enzymes in wheat plants treated with kartolin-4 and exposed to water stress. It has also been used in a study to investigate the specific density of leaf as a characteristic of the photosynthetic apparatus.

Biochem/physiol Actions

Phospho(enol)pyruvate Carboxylase from corn was found to be highly susceptible to trypsin digestion.

Price and Availability

Suggested Laboratory Gloves


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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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