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  • P2069 - Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA.

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P2069 Sigma

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA.

for molecular biology

Properties

Related Categories Acids and Bases, Biochemicals and Reagents, Core Bioreagents, DNA & RNA Purification, Denaturation,
grade   for molecular biology
pH range   6.5 - 6.9 ( (pH of phenolic phase))
  7.8 - 8.2 ( (pH of phenolic phase after the addition of Equilibration Buffer))
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Application

For applications requiring a higher pH, such as the isolation of large intact genomic DNA, addition of the Equilibration Buffer is recommended.

Other Notes

After the addition of Equilibration Buffer, the product is stable for 6 months vs. 2 years without added buffer. Storage at -20 °C protected from light extends the shelf life to greater than 1 year after the addition of Equilibration Buffer.

Packaging

100, 400 mL in glass bottle

Supplied with P3803 and a separate bottle of equilibration buffer to adjust the phenol phase to pH 8.0 ± 0.2.

Suitability

This lot of phenol:chloroform:isoamyl alcohol 25:24:1 was use tested by extracting a crude preparation of pBR322 DNA preparation from E. coli. Based on evaluation by agarose gel electrophoresis the extracted DNA was not degraded or reduced in concentration. The same procedure was applied to purified pBR322 DNA and lambda Hind III digest DNA. Based on electrophoretic evaluation the purified preparations were not detectably degraded or reduced in concentration.

Price and Availability


Genotyping

RNAi Offer

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Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

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