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  • P2069 - Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA.

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P2069 Sigma

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA.

for molecular biology

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Properties

Related Categories Acids and Bases, Biochemicals and Reagents, Core Bioreagents, DNA & RNA Purification, Denaturation,
grade   for molecular biology
pH range   6.5 - 6.9 ( (pH of phenolic phase))
  7.8 - 8.2 ( (pH of phenolic phase after the addition of Equilibration Buffer))
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Application

For applications requiring a higher pH, such as the isolation of large intact genomic DNA, addition of the Equilibration Buffer is recommended.

Other Notes

After the addition of Equilibration Buffer, the product is stable for 6 months vs. 2 years without added buffer. Storage at -20 °C protected from light extends the shelf life to greater than 1 year after the addition of Equilibration Buffer.

Packaging

100, 400 mL in glass bottle

Supplied with P3803 and a separate bottle of equilibration buffer to adjust the phenol phase to pH 8.0 ± 0.2.

Preparation Note

Add the entire contents of the small bottle of Equilibration Buffer to the large bottle of Phenol:Chloroform:Isoamyl Alcohol. Mix gently and allow the phases to separate before use, approximately 2-4 hours. This will adjust the pH of the Phenol solution from pH 6.7 ± 0.2 to 8.0 ± 0.2.

Suitability

This lot of phenol:chloroform:isoamyl alcohol 25:24:1 was use tested by extracting a crude preparation of pBR322 DNA preparation from E. coli. Based on evaluation by agarose gel electrophoresis the extracted DNA was not degraded or reduced in concentration. The same procedure was applied to purified pBR322 DNA and lambda Hind III digest DNA. Based on electrophoretic evaluation the purified preparations were not detectably degraded or reduced in concentration.

Price and Availability


Genotyping

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Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN 2810 6.1 / PGIII
WGK Germany 
3

Protocols & Articles

Protocols

Reverse Transcription Using ReadyScript cDNA Synthesis RDRT Protocol

Preparation Place components on ice. Mix, and then briefly centrifuge to collect contents at the bottom of the tube

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