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  • P2069 - Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA

P2069 Sigma

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA

Supplied with Equilibration buffer, for molecular biology



Related Categories Acids and Bases, Biochemicals and Reagents, Core Bioreagents, DNA & RNA Purification, Denaturation,
grade   for molecular biology
pH range   6.5 - 6.9 ((pH of phenolic phase))
  7.8 - 8.2 ((pH of phenolic phase after the addition of Equilibration Buffer))
Featured Industry   Agriculture
storage temp.   2-8°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

After the addition of Equilibration Buffer, the product is stable for 6 months vs. 2 years without added buffer. Storage at -20 °C protected from light extends the shelf life to greater than 1 year after the addition of Equilibration Buffer.


100, 400 mL in glass bottle

Supplied with P3803 and a separate bottle of equilibration buffer to adjust the phenol phase to pH 8.0 ± 0.2.

Preparation Note

Add the entire contents of the small bottle of Equilibration Buffer to the large bottle of Phenol:Chloroform:Isoamyl Alcohol. Mix gently and allow the phases to separate before use, approximately 2-4 hours. This will adjust the pH of the Phenol solution from pH 6.7 ± 0.2 to 8.0 ± 0.2.


For applications requiring a higher pH, such as the isolation of large intact genomic DNA, addition of the Equilibration Buffer is recommended.

Phenol:Chloroform:Isoamyl Alcohol has been used in the preparation of RNA for the study of total RNA isolation from myocardial biopsies. It has been also used to extract DNA during Human Androgen Receptor (HUMARA) Clonality Assay.


This lot of phenol:chloroform:isoamyl alcohol 25:24:1 was use tested by extracting a crude preparation of pBR322 DNA preparation from E. coli. Based on evaluation by agarose gel electrophoresis the extracted DNA was not degraded or reduced in concentration. The same procedure was applied to purified pBR322 DNA and lambda Hind III digest DNA. Based on electrophoretic evaluation the purified preparations were not detectably degraded or reduced in concentration.

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Safety & Documentation

Safety Information

Signal word 
UN 2810 6.1 / PGIII
WGK Germany 
Protocols & Articles


Reverse Transcription Using ReadyScript cDNA Synthesis RDRT Protocol

Preparation Place components on ice. Mix, and then briefly centrifuge to collect contents at the bottom of the tube

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