P2192 Sigma

10X PCR Buffer

Optimized for routine PCR with MgCl2 included



Related Categories Molecular Biology, PCR Reaction Components, PCR/Amplification More...
Quality Level   PREMIUM
form   liquid
packaging   vial of 1.5 mL
color   colorless
Featured Industry   Agriculture
foreign activity   DNase, RNase, none detected
storage temp.   −20°C



Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin


For routine PCR amplifications

Features and Benefits

• Standalone buffer
• Compatible with JumpStart Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Discover the Milli-Q® IQ 7000 ultrapure water system
Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Amplification of DNA Using Jumpstart REDTaq DNA Polymerase D8187 Protocol

Note: The use of DMSO or formamide with JumpStart REDTaq DNA Polymerase is not recommended due to interference with the enzyme-antibody complex. Other co-solvents, solutes (e.g., salts) and extremes ...
Keywords: Amplification, Evaporation, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Agriculture | Plant Breeding Workflow

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers


Set your institution to view full text papers.
Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?