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P2192 Sigma

10X PCR Buffer

Optimized for routine PCR with MgCl2 included

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Properties

Related Categories Molecular Biology, PCR Reaction Components, PCR/Amplification More...
Quality Level   PREMIUM
form   liquid
packaging   vial of 1.5 mL
color   colorless
Featured Industry   Agriculture
foreign activity   DNase, RNase, none detected
storage temp.   −20°C

Description

Components

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

Application

For routine PCR amplifications

Features and Benefits

• Standalone buffer
• Compatible with JumpStart Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Ultrapure dNTPs for PCR

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Protocols

Amplification of DNA Using Jumpstart REDTaq DNA Polymerase D8187 Protocol

Note: The use of DMSO or formamide with JumpStart REDTaq DNA Polymerase is not recommended due to interference with the enzyme-antibody complex. Other co-solvents, solutes (e.g., salts) and extremes ...
Keywords: Amplification, Evaporation, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

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Peer-Reviewed Papers
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Description

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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