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P2265 Sigma

Phosphatase, Alkaline from rabbit intestine

0.2-0.6 unit/mg solid (in glycine buffer)

Synonym: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Description

Analysis Note

Protein determined by biuret.

Unit Definition

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 10.4 at 37° C.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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inhibitor

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Description

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D6518 Diethylenetriaminepentaacetic acid, ≥99% (titration)
P9375 1,10-Phenanthroline monohydrate, reagent grade
E4378 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, ≥97.0%
ED2SS Ethylenediaminetetraacetic acid disodium salt dihydrate, reagent grade, 98.5-101.5% (titration)

substrate

Product #

Description

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N2770 SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL
N1891 SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL
M3168 4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid

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