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P2317 Sigma

10X PCR Buffer without MgCl2

Optimized for routine PCR without MgCl2

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Properties

Quality Level   PREMIUM
form   liquid
color   colorless
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

Application

10× PCR Buffer without MgCl2 has been used with Sigma′s PCR enzymes.

General description

10X PCR Buffer II was tested at a final concentration of 1X (10mM Tris-HCl, pH 8.3 at 25 °C, 50mM KCl), in reactions containing 1-4mM MgCl2, each dNTP at 200 μM, primers defining an approximately 500 base pair region of λ DNA at 1.0μM each, λ DNA template at 1ng/100μL, and Taq DNA polymerase at 2.5 units/100μL. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments and 72 °C to extend the DNA segments. Following electrophoresis of the reaction products in 1.5% agarose gel, a single band of approximately 500 base pairs was visualized for PCRs containing 1-1.5mM MgCl2.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
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Protocols

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Peer-Reviewed Papers
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References

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